Nyquist Calculator: online calculator to estimate the optical slice thickness and optimal step size for your instrument will also calculate the point spread function (PSF) for your microscope.FPBase Fluorescent Protein Database (compare properties, look at 'evolutionary'/development lineages.Spectra and properties of fluorescent molecules and proteins These sheets also list pixels sizes, which are necessary for creating scale bars and performing spatial measurements. Microscope Objective and Filter Specification Sheets Want to know more about how to work with your images in ImageJ? See the information, below, in Image Processing and Analysis.Close the old image and work with the new one. If you have a true-color image (NOT fluorescence) and it displays as three separate red, green, and blue images overlayed in a single window with a slider at the bottom, go to the Image menu, select Type, then RGB Color.If a Console window also opens and lists warnings, ignore them. Select the images you want to open and click OK. Leica (.lif) Project files: After you hit OK in the previous menu, the Bio-Formats Series Options window will display.Some file formats may require additional steps.There are many useful options in this menu, but unless you know what they do, I recommend using these basic options: The Bio-Formats Import Options menu will display.Alternatively, go to Plugins > Bio-Formats > Bio-Formats Importer and select the file to open. Most image files can be opened by simply dragging and dropping the file onto the ImageJ toolbar.

Before you start using ImageJ, take a quick look through this webpage to orient yourself to the ImageJ program. If you already have ImageJ installed, you can install the Bio-Formats Package of plugins from the Open Microscopy Environment project to allow ImageJ to open most major proprietary image formats. Uncompress the downloaded file and you're ready to go! Just double-click on the fiji.app file. To install FIJI, click on the correct link for your operating system and save the file anywhere convenient (except in your Program Files folder). You need to install the FIJI version of ImageJ, which comes pre-configured with lots of useful plugins. ImageJ is a free, open-source image processing and analysis software and will work on nearly any operating system. The rest of this section explains how to use ImageJ to open images and later sections provide resources for learning how to use this powerful program for image processing and analysis. If you want to do image processing/analysis or the above options don't work for you, I recommend using ImageJ.If you acquired them on one of the Leica scopes, you can download a free viewing-only version of the Leica LAS software here (for Windows only).If you acquired them on one of the Nikon scopes, you can download a free viewing-only version of the Nikon Elements software here.You just want to see your images, adjust the brightness, export them as JPGs for a presentation, etc.Unless you are collecting basic 'screen-shot' quality images (jpgs), you will not be able to open your images using the basic software included in Windows and Mac OS. Online microscopy references and tutorials.Spectra and properties of fluorescent molecules.Microscope objective and filter specification sheets.

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